何化（副教授）

  姓    名：

何化

  职    称：

　副教授

  导师类别：

　硕士研究生导师

  系    室：

生物与能源化工系

  研究领域：

　分析化学

  电子邮箱：

huahe@upc.edu.cn

  联系电话：

0532-86981135

◎教育背景

1998-2002， 湖南师范大学MK体育，理学学士

2002-2005， 湖南师范大学MK体育，理学硕士

2005-2008， 上海交通大学MK体育，工学博士

2014-2015， 美国斯坦福大学MK体育，国家公派访问学者

◎工作经历

2008-2010，MK体育，讲师

2010-至今，MK体育，副教授

◎研究方向

1)    单分子荧光成像、超分辨荧光成像及人工智能技术的研究及应用

2)    重大疾病生物标志物和环境中微塑料等新型污染物的高灵敏检测

◎科研项目

1）国家自然科学基金面上项目（22574172），《基于荧光闪烁指纹智能识别的活细胞β-分泌酶活性超分辨成像》，主持

2）国家自然科学基金面上项目（21874154），《细胞膜上G蛋白偶联受体聚集与分布的荧光闪烁碳点超分辨成像》，主持

3）国家自然科学基金青年项目（20905078），《基于RGD配体设计的CdTe量子点靶向探针的直接合成及其应用》，主持

4）山东省自然基金面上项目（ZR2023MB010），《基于碳点闪烁的单分子定量超分辨成像新方法及其应用》，主持

5）山东省自然基金面上项目（ZR2014BM028），《荧光闪烁量子点用于细胞中趋化因子受体CCR3的三维超分辨定位与分布研究》，主持

6）山东省自然基金青年项目（ZR2009BQ001），《基于RGD肽的量子点靶向探针制备新方法研究及应用》，主持

7）中央高校基本科研业务基金（18CX02126A），《荧光碳纳米材料的制备及其在生物成像中的应用》，主持

8）中央高校基本科研业务基金（12CX04053A），《单个量子点的光学行为、调控及其应用研究》，主持

9）国家重点研发计划（2019YFA0708703），《超浸润智能驱油体系定向输送机制及智能诱导体系研发》，项目骨干，任务2：“智能诱导材料分子设计”

10）         山东省重大研发计划项目（2015GSF118013），《用于重大恶性肿瘤早期预警的等离子体纳米镀膜检测芯片关键技术研究》，第1参加人，承担任务：纳米镀膜芯片的制备技术及荧光增强研究。

11）         海关总署科技项目（2022HK058），《进口原油中非法添加物的识别与安全监管体系研究》，第1参加人，承担任务：用于红外光谱智能识别的深度学习算法开发

12）         海关总署科技项目（2017IK236），《纳米镀膜等离子体芯片用于石油产品中有毒有害物质的高通量检测研究》，第1参加人，承担任务：纳米镀膜芯片的制备技术及荧光增强研究

13）         青岛市自然科学基金重点项目（24-8-4-zrjj-9-jch），《基于分子工程调控的荧光碳量子点精准合成、发光机制及免疫层析应用研究》，第1参加人，承担任务：碳量子点合成及发光机制解析。

◎代表性论文及专利

以第一作者/通讯作者在Science   Advances、Angew. Chem.、Nano Lett.、Anal. Chem.、ACS Sensors等权威刊物发表论文40余篇, H因子25。

1)                 Shenming   Wang, Xiaohui LiuLin Yang, Wenxuan Yang, Zhenzhen Feng, Guangyong Qin, Tongtao   Yue, Hua He*, Jingbin Zeng*. Gap-Enhanced   Catalysis in Gold Nanostructures by Electric Field and Curvature Effects. Nano Letters2025, 25, 14, 5663–5671.

2)          Guangyong Qin, Xin Guan, Jian Mao, Zhenzhen Feng, Wenxuan Yang,   Shenming Wang, Zheng Zhen, Xintong Miao, Yifeng Cheng, Zhirui Wang, Xiaojuan   Wang, Fang Huang, and Hua He*. Nanosilver-Enhanced Far-Field Fluorescence   Fluctuations for Super-Resolution Microscopy. Nano Letters, 2024. 24, 15186−15194.

3)          Jian Mao, Hua He*. Deep Learning in Fluorescence Imaging and   Analysis. Journal of Intelligent Medicine 2024. 1(1), 42-62. (Invited Review)

4)          Hua He,* Guangyong Qin, Minmin Xue, Zhenzhen Feng, Jian Mao, Wenpeng Tao, Hongqi   Chen, Xiaojuan Wang, Daoyong Yu, and Fang Huang*. Mitochondrial Nanoprobe for   Precise Cellular and Drug Analysis via Graph Neural Network. Chemical   Engineering Journal 2024, 152709.

5)          Wenxuan Yang, Shenming Wang, Xiaoyun Wei, Zhenzhen Feng, Guangyong Qin,   Jian Mao, Zheng Zhen, Xintong Miao, Yifeng Cheng, Xiaojuan Wang, Fang Huang and   Hua He*,Ligand-Regulated   Catalytic Activity in Fluorescent Gold Nanoclusters. Inorganic   Chemistry Frontiers, 2024,   DOI: 10.1039/D4QI01828A  

6)          Hongqi Chen*, Ruoxin Wang, Juanjuan Cheng, Lun Wang and Hua He*. A Ratiometric Upconversion   Nanoprobe Enables Super-Resolution Imaging Sensing of Biothiols in Living   Cells. Chemical Communications 2024, 60(33), 4447-4450.

7)                 Jian Mao, Minmin Xue, Xin   Guan, Qian Wang, Zhirui Wang, Guangyong Qin, and Hua He*. Near-Infrared Blinking Carbon Dots Designed for Quantitative   Nanoscopy. Nano Letters,2023, 23,   1, 124-131.

8)          Hua He*, Guangyong Qin, Simin Bi,   Zhenzhen Feng, Jian Mao, Xin Guan, Minmin Xue, zhirui wang, Xiaojuan Wang,   Daoyong Yu, and Fang Huang*.Deep-Learning-Enhanced Diffusion Imaging Assay for   Resolving Local-Density Effects on Membrane Receptors. Analytical Chemistry, 2023,95(6), 3300-3308.

9)          Qian Wang, Qian Zhang, Hua He,* Zhenzhen Feng,   Jian Mao, Xiang Hu, Xiaoyun Wei, Simin Bi, Guangyong Qin, Xiaojuan Wang,   Baosheng Ge, Daoyong Yu, Hao Ren and Fang Huang*. Carbon Dot Blinking   Fingerprint Uncovers Native Membrane Receptor Organizations via Deep   Learning. Analytical Chemistry 2022, 94(9), 3914-3921.

10)      X. Mu#, J. Wang#, Hua He #, Qifeng Li, Bing Yang, Junhui   Wang, Haile Liu, Yalong Gao, Lufei Ouyang, Si Sun, Qinjuan Ren, Xinjian Shi,   Wenting Hao, Qiaoman Fei, Jiang Yang, Lulin Li, Ryan Vest, Tony Wyss-Coray,   Jian Luo, and Xiaodong Zhang*.An oligomeric semiconducting nanozyme with   ultrafast electron transfers alleviates acute brain injury.Science   Advances2021, 7(46), eabk1210.

11)      Hua He*, Xiaoliang Chen, Zhenzhen   Feng, Lihua Liu, Qian Wang, and Simin Bi.Nanoscopic Imaging of   Nucleolar Stress Enabled by Protein-Mimicking Carbon Dots. Nano Letters2021, 21(13),   5689–5696.

12)      Naseer Ullah Khan, Muhammad Zahir, Xukun Liu, Jing Lin, Qihong Zheng,   Huajie Zhang, Samiullah Malik, Hua He *, and Liming   Shen*. Ultra-sensitive detection of exosome using biofunctionalized gold   nanorods on silver-island film. Nano   Letters 2021, 21(13), 5532–5539.

13)      Qian wang, Hua He *, Qian Zhang, Zhenzhen Feng,   Jiqiang Li, Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong   Yu, Hao Ren and Fang Huang*. Deep-Learning-Assisted Single-Molecule Tracking   on a Live Cell Membrane. Analytical   Chemistry 2021, 93(25), 8810–8816

14)      Hua He, Lihua Liu, Xiaoliang Chen,   Qian wang, Xiaojuan Wang, Werner M. Nau*, and Fang Huang*.Carbon Dot Blinking   Enables Accurate Molecular Counting at Nanoscale Resolution. Analytical Chemistry 2021, 93(8),   3968–3975.

15)      Qian wang, Zhenzhen Feng, Hua He*, Jiqiang Li,   Xiaoliang Chen, Lihua Liu, Xiaojuan Wang, Baosheng Ge, Daoyong Yu, Hao Ren   and Fang Huang*.Nonblinking Carbon Dots for Imaging and Tracking Receptors on Live Cell   membrane. Chemical Communications 2021,   57(45), 5554-5557.

16)      Hua He*, Di Liu, Zhenzhen Feng,   Aijun Guo, Lihua Liu, and Xiaoliang Chen.Antifade Carbon Dots   on Plasmonic Substrate for Enhanced Protein Detection in Immunotherapy. ACS Sensors 2020, 5(12),   4027–4034.

17)      Hua He, Xinjian Shi, Junying Wang,   Xiaojuan Wang, Qian Wang, Daoyong Yu, Baosheng Ge, Xiaodong Zhang* and Fang   Huang*. Reactive Oxygen Species-Induced Aggregation of Nanozymes for Neuron   Injury. ACS Applied Materials &   Interfaces 2020,12(1), 209-216.

18)      Lufei Ouyang, Xiaoyu Mu, Junying Wang, Qifeng Li, Yalong Gao, Haile   Liu, Si Sun, Qinjuan Ren, Ruijuan Yan, Jingya Wang, Qiang Liu, Yuanming Sun,   Changlong Liu, Hua He*, Wei Long and Xiaodong Zhang*.   Carbon dot targeting to nitrogen signaling molecules for inhibiting neuronal   death. Journal of Materials   Chemistry B 2020,8(11), 2321-2330.

19)      Xiaoyu Mu#, Hua He#, Junying Wang#, Wei Long, Qifeng Li,   Haile Liu, Yalong Gao, Lufei Ouyang, Qinjuan Ren, Si Sun, Jingya Wang, Jiang   Yang, Qiang Liu, Yuanming Sun, Changlong Liu, Xiaodong Zhang*, Wenping Hu.   Carbogenic Nanozyme with Ultrahigh RNS Selectivity for Traumatic Brain   Injury. Nano Letters2019,   19(7), 4527-4534.

20)    Yuan He, Zhenzhen Feng,   Xinjian Shi, Shan Li, Yunguo, Liu*, Guangming Zeng, and Hua He*, N- and O-doped Carbon Dots for Rapid and High-Throughput Dual Detection   of Trace Amounts of Iron in Water and Organic Phases. Journal of Fluorescence 2019, 29(1):137-144.

21)      Naseer Ullah Khan, Hua He*, Xiaojuan Wang, Baosheng Ge,   Qian Wang, Xu Liu, Jun Lao, Yanan Wang, Jiqiang Li, Zhencai Wang, Shitan   Zhou, and Fang Huang*. A Two-Color Fluorescence Enhanced Dot-Blot Assay for   Revealing Co-operative Expression of Chemokine Receptors in Cells. Chemical Communications 2018, 54,   778-781.

22)      Hua He, Shan Li, Xinjian Shi,   Xiaojuan Wang, Xu Liu, Qian Wang, Aijun Guo, Baosheng Ge, Naseer Ullah Khan,   and Fang Huang, Quantitative Nanoscopy of Small Blinking Graphene   Nanocarriers in Drug Delivery. Bioconjugate   Chemistry2018, 29 (11), 3658–3666.

23)      Naseer Ullah Khan, Zhenzhen Feng, Hua He*, Qian Wang, Xu   Liu, Shan Li, Xinjian Shi, Xiaojuan Wang, Baosheng Ge and Fang Huang*, A   Facile Plasmonic Silver Needle for Fluorescence-Enhanced Detection of Tumor   Markers. Analytica Chimica Acta2018, 1040, 120-127.

24)    Lingfang Liu, Xiaoyu Mu,   Haixia Liu, Qian Wang, Xueting Bai, Junying Wang, Haile Liu, Fujuan Xu, Yaqi   Jing, Haitao Dai, Changlong Liu*, Hua He*, and Xiaodong Zhang   *, Structure, luminescence, and bioimaging of bimetallic CuAu nanoclusters. Optical Materials2018, 86,   291–297.

25)      Hua He, Xu Liu, Shan Li, Xiaojuan   Wang, Qian Wang, Jiqiang Li, Junying Wang, Hao Ren, Baosheng Ge, Shengjie   Wang, Xiaodong Zhang, Fang Huang, High-Density Super-Resolution Localization   Imaging with Blinking Carbon Dots. Analytical   Chemistry2017, 89 (21), 11831-11838.

26)      Jun Lao#, Hua He#, Xiaojuan Wang, Zhencai Wang, Yanzhuo   Song, Bin Yang, Naseer Ullahkhan, Baosheng Ge*, Fang Huang*. Single-Molecule   Imaging Demonstrates Ligand Regulation of Oligomeric Status of CXCR4 in   Living Cells. ‎ Journal of   Physical Chemistry B 2017, 121 (7), 1466-1474.

27)      Hua He, Zhencai Wang, Tiantian   Cheng, Xu Liu, Xiaojuan Wang, Junying Wang, Hao Ren, Yawei Sun, Yanzhuo Song,   Jiang Yang, Yongqing Xia, Shengjie Wang, Xiaodong Zhang*, and Fang Huang*.   Visible and Near-Infrared Dual-Emission Carbogenic Small Molecular Complex   with High RNA Selectivity and Renal Clearance for Nucleolus and Tumor   Imaging. ACS Applied Materials &   Interfaces 2016, 8 (42), 28529-28537.

28)      Hua He, Xiaojuan Wang, Tiantian   Cheng, Yongqing Xia, Jun Lao, Baosheng Ge, Hao Ren, Naseer Ullah Khan, Fang   Huang*. An Ultra-High Fluorescence Enhancement and High Throughput Assay for   Revealing Expression and Internalization of Chemokine Receptor CXCR4. Chemistry-A European Journal 2016, 22, 5863-5867.

29)      Hua He *, Xiaojuan Wang, Zhenzhen Feng, Tiantian Cheng, Xing Sun, Yawei Sun,   Yongqing Xia, Shengjie Wang, Junying Wang, Xiaodong Zhang*. Rapid   microwave-assisted synthesis of ultra-bright fluorescent carbon dots for live   cell staining, cell-specific targeting and in vivo imaging. Journal of Materials Chemistry B2015,   3, 4786-4789.

30)      Hua He, Xing Sun, Xiaojuan Wang,   Yawei, Sun, Hai Xu*, Jian R. Lu*. Cyclic Arginyl-Glycyl-Aspartic Acid (RGD)   Peptide-Induced Synthesis of Uniform and Stable One-Dimensional CdTe   Nanostructures in Aqueous Solution. RSC   Advances 2014, 4, 11794-11797.

31)      Hua He*, Xing Sun, Xiaojuan Wang and Hai Xu*,   Synthesis of Highly Luminescent and Biocompatible CdTe/CdS/ZnS Quantum Dots   Using Microwave Irradiation: a Comparative Study of Different Ligands, Luminescence 2014, 29, 837–845.

32)      Hua He, Min Feng, Jing Hu, Cuixia   Chen, Jiqian Wang, Xiaojuan Wang, Hai Xu*, Jian R. Lu*. Designed Short RGD   Peptides for One-Pot Aqueous Synthesis of Integrin-binding CdTe and CdZnTe   Quantum Dots. ACS Applied Materials   & Interfaces2012, 4, 6362-6370.

33)      Hua He, Chao Xie, Jicun Ren*.   Non-Bleaching Fluorescence of Gold Nanoparticles and Its Applications in   Cancer Cell Imaging. Analytical   Chemistry2008, 80(15): 5951-5957.

34)      Hua He, Jicun Ren*. A Novel   Evanescent Wave Scattering Imaging Method for Single Gold Particle Tracking   in Solution and on Cell Membrane. Talanta2008, 77(1):166-171.

35)      Hua He, Huifeng Qian, Chaoqing   Dong, Kanglin Wang, Jicun Ren*. Single nonblinking CdTe quantum dots   synthesized in aqueous thiopropionic acid. Angewandte Chemie International Edition 2006, 45(45): 7588-7591.   

36)    Hua He, Qingji Xie, Shouzhuo Yao*. An electrochemical quartz crystal   impedance study on anti-human immunoglobulin G immobilization in the polymer   grown during dopamine oxidation at an Au electrode.Journal of Colloid and Interface Science2005, 289, 446-454.

37)      Hua He, Qingji Xie, Youyu Zhang,   Shouzhuo Yao*. A simultaneous electrochemical impedance and quartz crystal   microbalance study on antihuman immunoglobulin G adsorption and human   immunogloulin G reaction. J.   Biochem. Bioph. Meth.2005, 62, 191-205.

国家发明专利：

1)          一种双效荧光增强的免疫分析方法, 专利号：ZL 202010520800.1

2)          一种受自由基诱导聚集的治疗性纳米酶及其制备方法，专利号：ZL201910660001.1

3)          一种基于免疫荧光增强的外泌体定量检测方法, 专利号：ZL202110648316.1

◎代表性著作

1)          Hua   He,   Qian Wang, Xiaoqiang Wang, Fang Huang. Single molecule fluorescence for   studying the membrane protein dynamics and interactions, in: Biomembranomics:   Structure, Techniques, and Applications, ‎Jenny Stanford   Publishing 2024, Chapter 6, 201-237. 受邀撰写

2)          Hua   He,   Xiaojuan Wang, Fang Huang. Analysis and Applications of Single Molecule   Fluorescence in Live Cell Membranes, in: ‎Membrane Biophysics: New Insights   and Methods, ‎Springer2018, Chapter 6, 147-173.   受邀撰写

◎获奖情况及荣誉称号

2018年青岛市自然科学奖三等奖   

◎执教课程

研究生： 《生物仪器分析技术》、《现代分析科学》、《生物材料》；职工英文课程《仪器分析技术及应用》

本科生：《分析化学》、《分析化学实验》、《仪器分析实验》、《无机及分析实验》、《现代分析技术》、《生物信息学与人工智能》

◎招收及指导研究生情况

1.    指导研究生情况：

共指导博士和硕士研究生30余名，在读12名。毕业生中1名到卡塔尔气候部任研究科学家，1名到西安石油大学MK体育担任讲师，多名毕业生在国内知名高校攻读博士学位，其他毕业生在药明生物、药明康德、齐鲁制药、青岛卫生健康局等企业或事业单位工作。

2. 指导员工获奖：

2023年度校级优秀硕士学位论文（2020级硕士研究生毛健）

3. 招生专业及要求

生物化工、生物与医药、生物技术与工程

◎学术兼职

Frontiers in Chemistry副主编；

山东省物理学会生物物理专委会副秘书长；

国家自然科学基金委评议人；

中国化学会高级会员；

中国光学工程学会会员。